Adsorption study of methicillin onto innovative synthetized organosilicate particles
Alice Dufour1,§, Marc Legendre1,§, Camille Tonnin1,§, Saddam Charaabi1,2, Marc Le Borgne2, Anne-Marie Pensé-Lhéritier1 and Samar Issa1
1 Ecole de Biologie Industrielle EBI, EBInnov®
49 avenue des Genottes CS90009
95895 Cergy-Pontoise, France
2 Université de Lyon, Université Lyon 1, Faculté de Pharmacie – ISPB, EA 4446 Bioactive Molecules and Medicinal Chemistry, SFR Santé Lyon-Est CNRS UMS3453-INSERM US7 8 avenue Rockefeller
69373 Lyon cedex 8, France
Abstract. In this study, innovative hydrophilic organosilicate particles (OSH) were obtained and their capacity to adsorb an antibiotic, methicillin (Met), was investigated. The effect of the main experimental parameters was evaluated: solvent, temperature, pH, methicillin initial concentration and contact time. The adsorption capacity of OSH was evaluated by High Performance Liquid Chromatography (HLPC) coupled to UV detection at 238 nm. The OSH-Met complexes were characterized by means Attenuated Total Reflectance – Fourier Transform InfraRed (ATR – FTIR) spectroscopy. Adsorption isotherm models (Langmuir, Freundlich and Temkin) and kinetic models (pseudo-first-order, pseudo-second order and intraparticle diffusion) were studied. The optimal batch adsorption was conducted in ethanol, pH 7, 40 °C, with an initial concentration of methicillin of 200 mg L-1 for 300 min (5 h). The adsorption capacity of OSH for Met followed both the Langmuir adsorption isotherm model and the pseudo-second order kinetic model.
Keywords: Organosilicate particles, Methicillin, Adsorption isotherm, Pseudo-second order